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Effect of combinatorial therapies on blood plasma parameters. Blood plasma from female ( A , C , E ) and male ( B , D , F ) mice was analyzed for AST activity ( A , B ), creatinine concentrations ( C , D ), and C-peptide levels ( E , F ) following treatment with the BI-3406 (BI) and trametinib (T) combination therapy with or without BKM120 (BKM). Shaded areas indicate the range observed in healthy C57BL/6 mice (n = 5). Statistical analyses were performed using an unpaired t-test ( C – E ) or Mann–Whitney test ( A , B , F ). *p < 0.05

Journal: BMC Cancer

Article Title: Benefits and challenges of adding BKM120 to a BI-3406 plus trametinib combination therapy

doi: 10.1186/s12885-026-16409-0

Figure Lengend Snippet: Effect of combinatorial therapies on blood plasma parameters. Blood plasma from female ( A , C , E ) and male ( B , D , F ) mice was analyzed for AST activity ( A , B ), creatinine concentrations ( C , D ), and C-peptide levels ( E , F ) following treatment with the BI-3406 (BI) and trametinib (T) combination therapy with or without BKM120 (BKM). Shaded areas indicate the range observed in healthy C57BL/6 mice (n = 5). Statistical analyses were performed using an unpaired t-test ( C – E ) or Mann–Whitney test ( A , B , F ). *p < 0.05

Article Snippet: Blood chemistry parameters, such as aspartate aminotransferase (AST), and creatinine were measured using a cobas c111 system (Roche Diagnostics, Mannheim, Germany), and C-peptide levels were quantified with a mouse C-peptide ELISA kit (ALPCO, Salem, USA) according to the manufacturer’s instructions.

Techniques: Clinical Proteomics, Activity Assay, MANN-WHITNEY